11/8/2023 0 Comments Flowjo biexponential scalingPreviewing and PDF creation can be done within the BD FACSDiva™ Software interface.īD FACSDiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. Snap-to grids allow for easy alignments of plots, comments and statistics. Worksheets are configurable using predefined and customizable headers and footers. These include gating tools to automatically find a population, biexponential scaling to completely see populations that might have negative relative fluorescence intensities, and overlays to compare multiple tubes or samples. NOTE: The creation of customized configurations is not available for the FACSCanto family flow cytometers.īD FACSDiva™ Software provides a wide array of basic analysis features enabling multiple applications to run on the BD FACSCanto™, BD ® LSR and BD FACSAria™ Flow Cytometers. ![]() This allows users to select the correct configuration for their work.Īdministrators can create– for BD FACSCelesta™, BD FACSAria™ II, III and BD FACSCAria™ Fusion, for BD ® LSR II, BD LSRFortessa™ and BD LSRFortessa™ X-20, specialized configurations for particular applications by dragging filters, mirrors and fluorochromes onto a representation of the optical bench. Note: refer to the BD FACSDiva Software Reference Manual and the BD Cytometer Setup and Tracking Application Guide for more details on how to create baseline, performance checks and application settings (upgrading from FACSDiva v6.x to v8.0 causes loss of existing baseline and application settings)īD FACSDiva™ Software provides a graphic of the cytometer's physical optical configuration, showing the layout of detectors, filters and mirrors. Out of boundary results are flagged for the user to quickly identify potential problem areas. This automation reduces startup time to approximately five minutes and eliminates multiple error-prone and expensive data acquisitions and calculations.īaseline and daily performance reports are automatically generated and organized for easy recall. Key cytometer setup values for PMT voltages, laser delay and area scaling factors are automatically calculated and adjusted to maintain optimal performance levels over time. PMTV, Qr, Br, Bright Bead Target Value, Bright Bead %rCV, Mid Bead Median, Mid Bead %rCV, Dim Bead Median, Dim Bead %rCV, Forward Scatter Laser Delay, Fluorescence Laser Delay, Laser Current, Laser Power, Fluidics Pressureĭefine Baseline, Check Performance, Auto Compensation, Reset Target ValuesĮxperiments, FCS Files, Statistics, Templates Left, Right, Bottom, Top, Distribute Horizontally, Distribute Vertically Histogram, 2D Dot Plot, 2D Contour Plot, 2D Density Plot Robust Standard Deviation, Robust Percent Coefficient of Variation ![]() Minimum, Maximum, Geometric Mean, Mean, Median, Standard Deviation, Percent Coefficient of Variation, Mode Interval, Rectangle, Polygon, Quadrants, Hinged QuadrantsĪuto-Polygon, Auto-Interval, Snap-To Polygon, Snap-To Interval User-Defined Event Number, User-Defined Percentage U, V and flat-bottom 96-well plates, flat-bottom 384-well plates, Terasaki plates (BD FACSAria™ Cytometer only)ġ45 (See Table 1 in Qr and Br in BD FACSDiva™ Software: Parameters for Characterizing Detector Performance) BD FACSCanto™ 10-color, BD FACSCelesta™, BD FACSCanto™, BD FACSCanto™ II, BD FACSAria™ II, BD FACSAria™ III, BD FACSAria™ Fusion, BD ® LSR II, BD LSRFortessa™, BD LSRFortessa™ X-20, BD FACSymphony™ S6, BD FACSymphony™ A3 and BD FACSymphony™ A5īD FACS™ Loader for the BD FACSCanto™ platforms, BD ® High Throughput Sampler for the BD FACSCanto™ and BD ® LSR platforms, and BD FACS™ Accudrop for the BD FACSAria™ platform
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